The Greeneville Astros are a minor league baseball team of the Appalachian League, and a Rookie-level affiliate of the Houston Astros. They are located in Greeneville, Tennessee, and play their home games at Pioneer Park on the campus of Tusculum College.

The Astros have led the Appalachian League in average attendance every year since their move to Pioneer Park.

In 2007, the first former Greeneville Astros made it to the major leagues. Four players from the 2004 Greenville team were called up to Houston late in the 2007 season: Juan Gutierrez, Felipe Paulino, Troy Patton, and J. R. Towles.

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